Protocols

Enhanced Two-Color Fluorescent In Situ Hybridization for Low-Abundance Genes

Click here for the complete list of DOR proteins in Vosshall et al.,1999; Vosshall et al., 2000.

Preparation of Tissue for Larval In Situ Hybridization (Frozen Sections)

Single Fly Genomic DNA Protocol

ABI Prism 310 Cycle Sequencing Protocol

ABI Prism 310 Cycle Sequencing Protocol – Economy Version

Bacterial Transformation

Buck Buffer

Complex cDNA Probes

Drosophila Embryo Microinjection

Egg Collecting Plates

Whole Mount Drosophila Embryo In Situ Hybridization with RNA probes

Whole Mount Fluorescent Antibody Staining of Drosophila Embryos

Expand High Fidelity PCR Reactions

Fixing Embryos for In Situ Hybridization

Making FIXATIVE for In Situ and Antibody Staining

Drosophila Genomic DNA Preparation

Genomic Southern Protocol

Hanahan Competent Cell Protocol

Immunofluorescence

Screening Bacteriophage Lambda Libraries

Whole Mount Fluorescent Antibody Staining of Drosophila Larvae

Mass Dechorionation for Embryo Microinjection

Partial Restriction Digests

Pharmacia Spin Column Protocol

Plasmid Colony PCR

Stocker Fly Brain Immunofluorescence Protocol

DIG In Situ Hybridization Protocol(with detergent)

X-GAL STAINING [Frozen Cryostat Sections]

X-GAL STAINING [Adult Head Whole Mounts]

Annotation, localization, and clone information for all DOR genes (Excel file for download) (PDF)

Inverse PCR & Cycle Sequencing of P Element Insertions for STS Generation

Fly Head Sections Using Martin Heisenberg Fly Collars

Fly Food Protocol - 1x

Drosophila Larval Chemotaxis Assays

This material is based upon work supported by the National Science Foundation under Grant No. 0092693; the National Institutes of Health Grant number RO1 DC05036. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation